Sperm chromatin stability and their relationship with fertilization rate in Sheep of the Junín race

The objective of this research was to evaluate the effect sperm on chromatin stability and its relationship with membrane integrity structural – physiological rate fertilization female sheep. Ejaculates (2 × 109 sperm·mL-1) 70% motility were collected using an artificial vagina (n=5, 2 years old. For this, each ram served fifteen sheep (n=75), generating thus five different Groups (A, B, C, D, E). A control Group also considered. Sperm nuclear (NCS) evaluated Borate Buffer (BB), Sodium Dodecyl Sulfate (SDS), mixture Ethylenediaminetetraacetic acid (EDTA) + SDS. after 16-18 hours post injection. concentration showed a significant difference (P<0.05) between Groups. In Contrast, seminal volume, do not show (P>0.05). high correlation (r2=0.52) observed morphology motility, 74.6% (n=56). It concluded in general that techniques condensation values have likelihood give diagnosis about future potential populations Junín ram.